1 What advantages do organelles provide to eukaryotic cells? Are they necessarily required or simply an artefact of the evolutionary history of eukaryotes? Why, for example, would eukaryotic cells require a membrane-bound nucleus when prokaryotes seem to replicate efficiently without one?

2. What are the 3 main intracellular transport mechanisms for proteins? How does cell topology influence the particular mechanisms needed to transport a protein among organelles intracellularly?

3. Here’s a chicken and egg question… how are transmembrane proteins added to membranes in newly synthesized organelles if there are no translocater proteins already present to direct them?