Tyrosinse
Project description
Forensic practical-Bodies, tissues and fluids
you only need two or three graphs; for part B you plot a graph of absorbance against time – all six lines can be on one set of axes. These might be straight lines or might reach a plateau which you should be familiar with from year one. determine the gradient of the line gives you the change in absorbance per minute if you do it sensibly; this needs to be initial rate.
you can now work out 1/V (the velocity NOT volume) by dividing 1 by change in absorbance per minute
you can work out 1/[S] because you know stock was 2mgml-1 and for example in cuvette 1 there was 1.5ml in total of 3 ml so concentration was 1 mgml-1. The RMM is given so you can convert to molarity – i’ll leave you to do that. Divide 1 by these figures to give 1/[S].
you can now do M-M kinetics by plotting 1/[S] on x- axis and 1/V on y-axis (you were shown this in year 1); you will have one point for each cuvette. This should give a straight line with an intercept on the x-axis of a negative value which is -1/Km.

For part C plot a graph of absorbance against time again and determine change of absorbacne per minute as above.
determine 1/V and 1/[S] as above; the substrate is still the same i.e. DOPA.
put the points on the graph and see what effect the inhibitor has on the kinetics. It should either change the x-axis intercept but not the y or vice versa. I will leave you to look at what happened and what should have happened but a search of Google will tell you if benzoic acid and thiourea is a competitive or non-competitive inhibitor of tyrosinase.
so you can now get on with the results and concentrate on background reading and putting together a good report in the required structure as outlined on BB and in the practical.
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