TRYSIN PURIFICATION BY AFFINITY CHROMATOGRAPHY AND SDS –PAGE TRYPSIN VISUALISATION

INTRODUCTION

Trypsin is among other three key digestive proteinases –pepsin and chymotrypsin-whereby

their main role is to break down complex protein molecules in to their simple forms; the amino

acids. For laboratory purpose trypsin is isolated from crude pancreas extract.

Affinity chromatography is a molecular used in separation and purification of generally all

biomolecules with regards of its chemical structure and biological function .Sepharose is maostly

used matrix where immobilization of ligand molecules are done. The ligand to be used should

have high affinity to the substrate- have specific and reversible binding sites and have chemically

modifiable group.