Transporting lactose in against concentration gradient

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April 22, 2020
Solution-Describe the general characteristics and structure
April 23, 2020

Transporting lactose in against concentration gradient

The lactose permease transporter of E. coli can accumulate lactose inside the cell by transporting lactose in against a concentration gradient, driven by a co-transport process in which the same protein brings protons into the cell from a higher concentration outside to a lower concentration inside.

A. What is the term used to describe the process (a type of active transport) in which one solute is transported against its concentration gradient by co-transport with another solute moving in the favorable direction? _______ active transport

B. If the proton gradient provided a driving force of –10 kJ/mol to support a maximum ?Gt of +10 kJ/mol for the lactose gradient, what would be the maximum lactose gradient (ratio of lactoseinside/lactoseoutside) that the cell could accumulate? Show your calculation.