The method of collection of immature oocytes can affect mitochondrial morphology and function

.

Follicle containing immature oocytes can be collected from ovaroan tissue by

Mechanical disaggregation

(use forceps and seisors

2. Enzyme digestion of the extra cellular matrix and connective tissue that the follicles embedded in

– collagenase – DNAse – Libarase – tryse

.

3. A combination of mitochondrial and enzyme disagrregation

Collect ovaries from naturally cycling mice culled in

routine anthiel hwse procedures

2. Dissect into rephiate small sections

3. Distribute to 4% paraformaldehyde fixation

.

H+E histology to quantity follicule

Mechanical disaggregation

Collagenase concentration 1, 2 & 3

Trypsin concentration 1, 2 & 3

Selected cllagenase concentration + selected hypsin concentration

sTo determine optomum time for each incubation

.