Solution-Stock of plasmid dna, You are ready to set up your
Concentration of ige fc fragments
April 28, 2020Identifying the organism as a land plant
April 28, 2020Solution-Stock of plasmid dna, You are ready to set up your
You are ready to set up your reaction. You are provided with a stock of plasmid DNA that is at a concentration of 1mg/ml. You have stock enzyme buffer that is 10X (meaning it is ten-times more concentrated than you need it to be in the actual reaction). You have deionized water (dH2O) to use, as needed.
- How many ml of DNA will you add to your reaction tube?
- How many ml of 10X buffer will you add to your reaction tube?
- How many ml of ApeKI will you add to your reaction tube?
- How many ml of dH2O will you add to your reaction tube?
- At what temperature will you incubate the reaction?
So how would you solve for these?
