Solution-Expression during sex determination in drosophila
To investigate transformer (tra) gene expression during sex determination in Drosophila, you perform in situ hybridization (FISH) and immunostaining. Fill in the table with the results you expect to observe following the example given in the first row.
|
Experiment |
Female embryos |
Male embryos |
|
Immunostaining with an antibody to Sxl protein |
stain positively |
no staining |
|
FISH with a probe to exon 2 of tra |
||
|
FISH with a probe to exon 3 of tra |
||
|
Immunostaining with an antibody to Tra protein |
Match sex phenotype to each genotype of a transformer mutant fly:
XX: ___________________________; XY: ________________________