BIOLOGY LAB REPORT

I have a biology experiment attached can write for me the introduction, Materials &

Methods and the conclusion

Introduction: Defines the subject, the purpose of doing the experiment; includes some background information (usually can be gathered from the experiment’s intro); reader should be able to understand why the experiment was done, what you already know about the experiment, and the specific purpose of the experiment (usually stated in the title). Do not plagiarize your introduction or you will lose points!!! Also, citing is more than copying the work and listing the source! You must cite within the text as well. Your entire introduction cannot be cited!!! You will lose points for intros that don’t have your original thoughts.

Materials & Methods: List the main materials used and write in your own words how the experiment is conducted; reader should understand what materials were used, how the materials were used, and what was actually done during the experiment. If the protocol was altered some way in class, you must make the changes in your lab report. Make sure you underline/italicize all microorganisms!!! Methods should be listed step by step vs. paragraph format.

Conclusion: answer questions at the end of the experiment, then discuss/conclude what your results mean in terms of the experiment and whether you were successful or not. The conclusion should not just state the experiment was successful, but definitive results should be explained here! Your conclusion should explain how the microbes used in the experiment were affected. For example, if the experiment is on gram staining, then your conclusion should state the gram reaction for each organism used. Ex: E.coli was determined to be gram negative, because it did not retain the purple of the primary stain, but retained the pink of the counter stain.

EXPERIMENT 1: Microscopic Examination of Living Microorganisms Using a Hanging-Drop Preparation or a Wet Mount

Review Questions

1. Why are living, unstained bacterial preparations more difficult to observe than stained preparations?

2. What is the major advantage of using living cell preparations (hanging-drop or wet mount) rather than stained preparations?

3. How do you distinguish between true motility and Brownian movement?

4. During the microscopic observation of a drop of stagnant pond water, what criteria would you use to distinguish viable organisms from nonviable suspended debris?

EXPERIMENT 2: Microscopic Examination Of Stained Cell Preparations

Review Questions

1. Explain why the body tube of the microscope should not be lowered while you are looking through the ocular lens.

2. For what purpose would you adjust each of the following microscope components during a microscopy exercise?

a. Iris diaphragm:
b. Coarse-adjustment knob:
c. Fine-adjustment knob:
d. Condenser:
e. Mechanical stage control:

3. As a beginning student in the microbiology laboratory, you experience some difficulties in using the oil-immersion lens. Describe the steps you would take to correct the following problems:

a. Inability to bring the specimen into sharp focus.
b. Insufficient light while viewing the specimen.
c. Artifacts in the microscopic field.

Attachment:- Experiment.rar