Q1. A patient who inherited two defective copies of a gene essential by white blood cells, suffered from a severe immune deficiency disease. The patient recovered from a disease following gene therapy, in that he received an expressed copy of the ‘missing’ gene (the desired outcome). Unfortunately, he afterwards developed leukaemia, a cancer involving the white blood cells (an unexpected outcome). The introduced gene was not responsible as it did not encode a carcinogen or a regulator of cell replication.

In a few sentences describe how the transformation might or DNA integration process has caused this outcome?

Q2. Autoimmune haemolytic anaemia is caused in general by auto anti bodies of the IgG and IgM classes. Can antibodies of other classes such as IgA, IgD, IgE cause autoimmune haemolytic anaemia?