Question: Assume you are provided an isolated muscle with its motor nerve intact. The preparation allows you to make electrophysiological measurements from the nerve and muscle as well as mechanical measurements of muscle contraction. You observe that if Drug A is added to the fluid bathing the preparation, the peak tension developed in a muscle twitch produced by stimulating the motor neuron is decreased
List all possible locations at which Drug A might be exerting its effect
Drug A is known to alter the transmembrane movement of cations. Which of the sites you listed above would fit this mechanism of action?
Suppose high concentrations of Drug A block muscle contraction completely in the presence of motor neuron stimulation. This effect is not prevented by high levels of either Ach or Ach- nicotinic receptor agonists. Now where do you think Drug A is acting? Justify your response.