Draw a restriction map of the plasmid
Solution-Bio 355 quiz, An 18-year-old female of Southeast
May 5, 2020You have a culture of these cells
May 5, 2020Draw a restriction map of the plasmid
Samples of a 4578 bp plasmid (i.e. circular DNA) is digested with the following enzymes and subjected to electrophoresis. The results are indicated in the below table.
Enzyme Resulting Fragment Sizes (in base pairs)
HindIII 4578
EcoRI 1851 and 2727
HindIII & EcoRI 437, 1851 and 2290
Draw a restriction map of the plasmid. Label the respective restriction sites (H and E for HindIII and Eco RI respectively) and indicate the distances between each of the fragments.
