Question: what are the 4 major macromolecules and list their building block, solubilty in water(hydrophobic/hydrophilic), and specific example of a polymer for each and where it is found(cell or organism).

Question: W1 should not have fluoresced. why? (I know it did not contain rGFP, but why?) W2-W4 should have fluoresced slightly. Give two possible reasons why. (yes, i know it contined GFP….. but why?)

Question: After you performed the freez-thaw cycle to lyse the bacteria, you pelleted the debris using a centrifuge. what two reasons did this pellet fluoresce green? (yes, I know it contained GFP, …… but why?)