Describe the effect of an uncoupling agent

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Describe the effect of an uncoupling agent

Mitochondria carrying out oxidative phosphorylation consume oxygen. a) What happens to oxygen in mitochondria? b) Describe the effect of an uncoupling agent such as 2,4-dinitrophenol on the rate of oxygen consumption. Assume there is a sufficient supply of oxidizable substrate, ADP, and Pi. c) It was observed that if a mitochondrial ATP synthesis inhibitor “compound X” is added to the cell, the NAD+/NADH ratio decreases. Would you expect compound X to be an uncoupling agent or an inhibitor of respiratory electron transfer? Explain briefly.