CRISPR/Cas9 KNOCKOUT technologies reveal role of ZNF703,CSMDI and HNF6 gene in colorectal cancer proliferation and metastits

Order Description
the proposal should have
abstract (200-250 words), the proposal body, objectives (three objectives) and methodology.

the proposal body is based on information in a paper attached files ( Al-Temaimi el at , 2016 + supplementary material)
proposal body:
the proposal should have a given cancer type being assessed but a property of all cancers. So for the introduction you need to generally describe what is cancer, what are the properties of cancer. Then focus on molecular aspects of these properties.
these are should be mention in the proposal.

the objectives should be as a 3-4 points .
and it includes knockout in corolectal cancer cells to estimated the relation between these genes and corolectal cancer proliferation, apoptosis and . metastasis

methodology:
corelectal cancer cell culture(cell culture)
CRSPER/CAS9 nickase single and multiple gene knock-out (addagene technology )
MTT Assay
proliferation apoptosis assay by flow cyclometery
realtime PCR
immunofluorescence microscopy
Bolting analysis
Invasion and migration assay

( note you can change the title for a better one, there will be some reference for introduction and methods attached)