QUESTION 1: Cell fractions and buffers (a) What factors determines the forces that a particle experience during centrifugation? (b) Name the techniques usually employed to obtain cell free extracts/”brake cells open”. (c) Indicate how mitochondria can be isolated by differential centrifugation. (d) Why is it important to know the pKa […]

Question 1 1. Choose the optimal enzyme concentration(s) observed.1. 50 uls2. 100 uls3. 200 uls Question 2 5. Based on the data at pH 2, what can you surmise happened to the active site?1. It stayed the same shape2. Its shape changed such that the substrate no longer fit inside3. […]

You place a sma-2 homozygous hermaphrodite and a wild-type (+) homozygous male on a Petri dish. Provide the phenotypic and genotypic ratios, showing all work, for an F(1) cross between these two C.elegans. Then provide your interpretation as to whether the mutation is dominant or recessive and a brief explanation […]

30. Proteins destined for secretion are first imported into the Golgi from the cytosol. (a) True (b) False 31. BiP is an HSP70 chaperone protein that binds proteins as they are entering the ER lumen, and assists folding and translocation. (a) True (b) False 32. An ER membrane protein that […]