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(Ligated Vibrio fischeri chromosomal DNA and pGEM plasmid vector with Sal I, and transformed E.coli cells with recombinant plasmids. And then, checked if the cloning was successful. Blue colonies indicate that it contains only the plasmid, and white colonies indicate that the plasmid and inserts are sucessfully transformed into the […]

1. Define evolution in the context of “Descent with Modification and understand the concept of the unity of life. 2. Understand the terms adaptation and natural selection and recognize Darwin’s contribution to our understanding of evolution. 3. Discuss the similarity and difference between natural selection and artificial selection 4. Discuss […]

An oligomeric protein has a molecular weight of 367000. A 460 mg sample was treated with an excess of 1-fluoro-2,4-dinitrobenzene (Sanger’s reagent) until the reaction was complete. Next the peptide bonds were hydrolyzed by heating with concentrated HCl. The product of the complete hydrolysis contained 2.2 mg of the compound […]

Martha brings her 11-year old grandson, James, to your clinic to have his right ear checked. He has complained to her about a mild earache for the past two days. His grandmother believes that he feels warm but did not verify this with a thermometer. James states that the pain […]