In the Yeast Two-Hybrid Screen technique, suppose I use a transcription factor in which the protein of the Activation Domain (AD) always binds the protein of the DNA Binding Domain (BD). What will happen in my Yeast Two-Hybrid experiments when I test for an interaction between Protein A and Protein […]

In a fern’s life cycle, of hybrid “1” the sperm in the antheridium was found to have a total of 8 chromosomes. On one of the chromosomes was the dominate gene “T” on one of the chromosomes which produced tall sporophyte fronds. In a hybrid “2” fern, the egg was […]

1) You are having a 2.5 mg/ml solution of an amino acid ( mol.wt. = 100). You are need to add the 0.5 µmoles into a test tube. Specify the amount you would pipette? 2) You require a 10mM solution of the potent (and dangerous) transcription inhibitor (mol.wt.500) to do […]

To assess which residues contribute most to the stability of a protein, mutants of the protein are made in which one residue is replaced by amino acid X. The stability of this mutated protein is then measured and compared to the original protein. By repeating the process for many different […]