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Unlike microscopy, cell fractionation and protein purification is often performed as a series of linked steps that can often take several days or longer than a week’s time. a. Most buffer solutions used in this process are isotonic and contain protease inhibitors. Why would this be the case? b. EGTA […]

1. Consider an atom of nitrogen (N), which is more electronegative than an atom of hydrogen (H). N has 5 valence electrons, whereas H only has 1 (H can only form 1 total pair of electrons in it’s valence shell) … Given this information: If an atom of N were to […]

You have screened for several new recessive mutations in a species of wasp. Wasps homozygous for apricot (aa) have pale orange eyes. (Wild-type eyes are brown.) Wasps homozygous for blunt (bb) have short wings. (Wild-type wings are long.) You make a pure-breeding double-mutant (apricot, blunt) line and cross it with wild-type wasps. The F1 is […]

Case Study: During a screening colonoscopy for a 40-year-old man, the doctor discovers a flat cancerous polyp in the transverse colon. Histopathology of the removed polyp indicated that cancerous cells were present in the margin, suggesting that some of the polyp remained behind in the colon. The doctor ordered an […]