Ethanol in the body is oxidized to acetaldehyde by liver alcohol dehydrogenase(LADH). Other alcohols are also oxidized by LADH. For example,methanol, which is mildly intoxicating, is oxidized by LADH to thequite toxic product of formaldehyde. The toxic effects of ingestingmethanol (a component of many commercial solvents) can be reducedby administering […]

Data suggest that rubisco (ribulose-1,5-bisphosphate carboxylase) makes up 10% of the total protein found in spinach leaves. Research elucidating the structure of rubisco shows that it has four active sites. Why, with four active sites, might there be such a large concentration of rubisco in plant matter? a.All heterotrophic organisms […]

A cross is made between 2 E. coli strains: Hfr arg+ bio+ leu+ (x) F- arg- bio- leu-. Interuppted mating studies show that arg+ enteres the recipient last, so arg+ recombinants are selected on a medium containing bio and leu only. The recombinants are tested for the presence of bio+ […]

(i) When constructing cDNA libraries it is very important to copy the whole of an mRNA into cDNA. One way to try and ensure that the 5′ end of a mRNA is represented in a cDNA copy is to use “cap-trapper” technology. Explain in your own words and with a […]