1. You clone a double stranded DNA fragment from a pathogenic fungal stain. You suspect that the fragment contains a gene encoding a Seven Amino Acid Peptide. Here’s one of the DNA strand fragments: 5′ T G A G A T G C T T A G C T G […]

lex A is an E. coli gene whose product regulates the transcription of certain target genes that are located at different positions in the bacterial chromosome. Identified target genes appear to be involved in DNA repair and recombination. Under normal conditions, the LexA protein binds near and represses transcription of target genes; however, when […]

1. Describe the general sequence of events in neuromuscular transmission at the neuromuscular junction. 2. What evidence supports a sliding filament-crossbridge mechanism of contraction in smooth muscle? 3. Present the comparison of neuromuscular blocking drugs and local anesthetics. What is a rationale to use them for pain control? How they […]

1. Assume you identify a recessive mutation in the AP endonuclease in yeast. During the diploid phase of their lifecycle, the yeast are viable but haploid strains die rapidly. Why might this be? 2. (a). What is the difference between germline and somatic mutations? (b) name at least one way that each […]