Although normal E.coli cells can synthesize all the amino acids, some mutants called amino acid auxotrophs, are unable to synthesize specific amino acids and require the addition of that amino acid to the culture medium for optimal growth. In addition to their role in protein synthesis, specific amino acids are […]

Q1. At the same time that you did the viable cell count of your bacterial culture (in problem 10), you also counted the cells in a Petroff-Hausser chamber; however, the cell count you obtained from Petroff-Hausser chamber is nearly double that obtained in the viable cell count. Based on this […]

Assume that you are working in a research lab and your professor asks you to make a solution of 50X TAE. She asks you to make 1 liter, containing 57.1 ml of glacial acetic acid as well as final concentrations of 2 molar (M) tris base and 0.05 M EDTA […]

Question #1: GPRCs and Cancer GPCRs and Cancer: The G-protein coupled receptor (GPCR) family of proteins boasts over 800 members, and more than 2% of the human genome codes for GPCRs. GPCRs are upstream of many of the regulatory signaling pathways discussed in this unit. The review by Dorsam and […]