An existing drug, OLD-STAT, is a competitive inhibitor of an enzyme that is too active in diabetes. The enzyme assay used to test and compare drug candidates is carried out at (S) = 0.15 mM. The enzyme has molecular weight of 28000, a turnover number of 13.8/sec, and the enzyme […]

Q1. In an exacting reaction X is converted to Y, with a free energy of -5.5 kcal/mole. Which molecule contains more energy, X or Y? Which molecule is steadier? At stability, will the concentration of X or Y be higher? If more “X” is added, will the reaction go to […]

Human populations are known to isolate themselves from other groups based on cultural identity, language religion, etc. Some of these communities in North America include the Ashkenazi (descendants of European Jewish communities, Yiddish speakers), the Cajun population of Louisiana, the Arcadian population of Canada, and the Old Order Amish community […]

1) Represent graphically the relation between melting temperature and the G-C content of DNA. a) Construct relations for dependence of melting temperature of a 30 bp DNA duplex on the G-C content. For series of the DNA chains where we vary the number of G-C pairs, plot how Tm changes […]