Q1. Genes A/a and B/b are linked and known to be 20 map units apart. Individuals heterozygous for both of these genes (genotypes= AB/ab) were mated with each other. If there 2000 offspring from this cross, how several of the offspring will you expect to show Ab or aB phenotypes? […]

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1. Why does Synechocystis not require electroporation or chemical pretreatment prior to transformation?2. What specific genetic manipulation does Synechocystis readily allow that E. coli does not?3. For transformation of Synechocystis, why do you first plate your transformant mixture on a filter on a BG – 11 plate with glucose, and […]

Mitochondrial ATP synthase comprises of several proteins, and some of these proteins are encoded by the genes in nucleus. One of these proteins is called S. A mutation in S gene deletes 21 base pairs which are immediately 3′ to the start codon. The mutant S gene is transcribed and […]