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Q. A eukaryotic gene has “sticky ends” produced by the restriction endonuclease EcoRI. The gene is additional to a mixture containing EcoRI and bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene. This […]

Vancomycinis an antibiotic that has become critical for controlling penicillin-resistant bacteria. This large glycopeptide (molecularwt. = 1449) binds to cellwall precursors and interferes with peptidoglycansynthesis. The polymyxinsare a group of antibiotics that are particularly effective as atopical antibiotic against skin infections. These largepolypeptides (molecular wt. = 1202) can disrupt some […]

Question: The plot of novelist Rose Darkly’s current work in progress, Despair at the Heat Death Cafe, revolves around the production of the “energy currency” of the cells of an alien species. Rather than have her aliens’ cells use ATP as the compound that links catabolism and anabolism, Ms. Darkly wants […]

Hematology Case studies The following case studies are not actual patients. They combine elements from different cases to emphasize important aspects Case 1 HISTORY: Patient Presentation A four-year-old African American male diagnosed with sickle cell disease in the newborn period was admitted to the hospital with abdominal pain. Two days […]