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Q. Your friend is trying to generate an in vitro system for studying the formation of COPII vesicles. He finds that to get well-organized vesicle budding from artificial membranes, he needs to add the coat proteins, the Sar1 GTP-binding protein, the GTPase-activating protein (GAP) for Sar1, and GMP-PNP (a nonhydrolyzable […]

Suppose that in your lab you occasionally get mice that have some sort of bizarre mutation-bent ears. You determine that the bent-ear trait is recessive. You then find out that someone else in your lab has isolated mice with bent ears as well and that they have also determined that […]

The following amino acid sequence was derived from the DNA sequence of a gene encoding a hypothetical type I integral plasma membrane protein. (The • merely indicate every 10 amino acids beginning with M1 & ending with V140). Use this information as necessary to answer questions 1-2.MGIIVLLLLW•VIALILAVAV•EKCPLLYINC•TRLSPQRTNY•SQRPLLFFWM•VILIVLALIF• LVIIVMPKKP• KDSHYRILVT•KHEDQNITEH•KRPDGEECTS•KRIANPTYCP•RKDELQLHDV•KRIANPLWCP• RKDELQLHDVWhat […]

Question1. Why is MgCl2 included in the PCR buffer? MgCl2 is used in the PCR buffer because it help Question2. If you started a PCR protocol with 10 double stranded molecular of DNA, how many double stranded molecules of product would you have after 5 cycles of PCR? Question3. Where […]