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A few amino acids are post-translationally removed from the C-terminal end of the beta-lactamase enzyme from B. imaginarium (i.e. after translation and release from the ribosome, a protease chews off a few amino acids). The pruified wildtype enzyme is 327 amino acids in length and the sequence of its C-terminal […]

A purified BamHI-cut DNA fragment is end-labeled (i.e. only the terminal 5′ phosphate from each strand is labelled) and partially digested with the enzyme DpnI. The reaction conditions were such that each DNA molecule, if cut at all, is cut only once. The resulting DNA fragments were electrophoresed on an […]

Question 1: Research 5-8 species within one Family of birds. Be sure to use primary or very good secondary literature for your information. Give a brief description of each species based upon the characteristics below. Construct a table(s) or cladogram(s) showing the similarity of those species in regard to the […]

Describe how you would identify E. coli mutants that cannot utilize galactose as a carbon (energy) source. You are given a test tube containing E. coli grown under full nutrition, a spreader, filter paper, Petri plates with agar gel containing various nutrients, and an incubator. There are two kinds of […]