E. coli chromosomes in which every nitrogen atom is labeled (that is, every nitrogen atom is the heavy isotope 15N instead of the normal isotope 14N) are allowed to replicate in an environment in which all the nitrogen is 14N. Using a solid line to represent a heavey polynucleotide chain […]

The gene that encodes the enzyme called tyrosine hydroxylase is known to be up regulated by the CREB protein.  Tyrosine hydroxylase is expressed in nerve cells and is involved in the synthesis of catecholamine, a neurotransmitter.  The expose of cells to adrenaline normally up regulates the transcription of the tyrosine […]

1. You require to prepare 200 uL of each protein at a concentration of 1mg/mL for an upcoming experiment. Calculate the recipe to make each desired solution at the above concentration and volume. Do you have enough materials to make each dilution? Note: Your diluent is water. a. Alpha-tubulin: you […]

Stress and the Cardiovascular and Digestive Systems Note: If you have trouble viewing some of the course materials, install Quicktime and the Adobe Shockwave Player, both of which can be downloaded free from the Internet. In this SLP we will investigate the effects of stress on the digestive system, including […]