A plasmid dna that was also cut with ecor i
Solution-Management chain intervened
April 28, 2020STR 581/ STR 581 Capstone Final Examination, Part 3 (Perfect Answer)
April 28, 2020A plasmid dna that was also cut with ecor i
The restriction enzyme ECOR I cuts DNA leaving 5′ overhangs, Pst I leaves 3′ overhangs after cutting DNA and SMA I leaves blunt ends on the fragments it produces.Indicate wheter DNA ligase can join the following pieces of DNA.
a) a fragment of human DNA that was generated by cutting with Ecor I and a plasmid DNA that was also cut with Ecor I .
b) a fragment of human DNA that was generated by cutting with Ecor I and a plasmid DNA that was cut with Pst I.
c) a fragment of human DNA that was generated by cutting with Sma I and a plasmid DNA that was also cut with Sma I.
